Journal: bioRxiv

Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

doi: 10.1101/2025.11.27.691042

Figure Lengend Snippet:

Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

doi: 10.1101/2025.11.27.691042

Figure Lengend Snippet: Modulation of paraxanthine accumulation by known CYP1A2 effectors in HepG2 and Hep3B cells. Paraxanthine concentration was quantified by LC–MRM. Data are shown as mean ± SD from three independent experiments. A-B, Sulforaphane reduced paraxanthine accumulation in HepG2 and Hep3B cells. C-D , Galangin increased paraxanthine accumulation in both cell lines. E-F , 3-Methylcholanthrene (3-MC) also increased paraxanthine accumulation in both cell lines. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Development of a New Approach Method to Monitor and Modify Caffeine Metabolism Correlated to CYP1A2 Expression

doi: 10.1101/2025.11.27.691042

Figure Lengend Snippet: qPCR measurement of CYP1A2 mRNA levels in HepG2 (top panels) and Hep3B (bottom panels) cells after 24-hour treatments with A-B , caffeine and caffeine along with C-D, D,L-sulforaphane, E-F , 3-methylcholanthrene, or G-H , galangin at the indicated concentrations. Control cells received the vehicle only. Data are normalized to a housekeeping gene and expressed as the mean ± SD (n = 3) from three independent experiments. The results of t-test are reported as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Human HCC cell lines Hep3B (HB-8064) and HepG2 (HB-8065) were purchased from ATCC (Manassas, VA, USA).

Techniques: Control

Journal: bioRxiv

Article Title: Super-resolved live imaging of thick biological samples with 3D Random Illumination Microscopy (3D-RIM)

doi: 10.1101/2025.08.06.668879

Figure Lengend Snippet: a : Schematic of the 3D-RIM set-up. The sample (here a FtsZ ring) is excited by a speckled illumination. A remote focusing unit, using an electrically tunable lens, permits to record z-stack images while keeping the illumination of the sample unchanged. Multiple (100 to 200) low-resolution 3D speckled images of a sample are recorded under different random speckled illuminations. The super-resolved 3D image is obtained from the variance of the speckled images using a variance matching algorithm (see the methods). b : FtsZ:GFP fusion in a live Streptococcus pneumonia bacterium during cell division. Schematic: FtsZ assembles into a ring that gradually shrinks as cytokinesis proceeds. XZ cuts of 3D images of closing rings at three different stages, obtained with 3D-RIM or deconvolved widefield microscopy. In sharp contrast with the deconvolved widefield images, the ring structure is well seen in the 3D-RIM images and the internal hole can even be suspected when the ring structure shrinks to a total diameter of 230 nm. c : A fixed human Hep3B cell stained for DNA.The sample is 15 microns thick. From left to right : one XY cut of the deconvolved widefield 3D image, the same cut imaged by 3D-RIM, the maximum intensity projection of the 3D-RIM image with colors encoding Z, see movie 1 for a 3D representation. The 3D-RIM resolution estimated by FRC is 110 nm transversally and 270 nm axially.

Article Snippet: Human hepatocarcinoma cells Hep3B (ATCC HB-8064) were seeded in μ -Dish 35 mm, high Grid-500 (ibidi ® , Cat 81166) at 250,000 cells per dish overnight in complete DMEM medium supplemented with 10% of SVF.

Techniques: Microscopy, Staining